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Position: Home >> Faculty >> Young Academic Leaders

Xiao Lehui

Subject: Analytical Chemistry

Address:Room 209, College of Chemistry, Nankai University, Tianjin


E-Mail: lehuixiao--at--163.com

Website: www.xiaolhlab.cn

Research Interest:
1) single molecule detection
2) synthesis of functional nanomaterials for biomedical applications
3) nanophotonics

Full Professor Nankai University, Nankai, Tianjin, China 2016.04-now
Full Professor Hunan Normal University, Changsha, Hunan, China 2013.01-2016-03
Assistant Professor Hunan Normal University, Changsha, Hunan, China 2012.09-2013.01
Postdoc. Research University of Washington, Seattle, WA, U.S.A. 2011.12-2012.09
Postdoc. Research Ames Lab., DOE, Ames, Iowa, U.S.A. 2011.09-2011.12
Ph. D. Hunan University&Iowa State University 2008-2011 Analytical Chemistry
M. S. Hunan University&HongKong Baptist University 2006-2008 Analytical Chemistry
B. S. Hunan University 2002-2006 Chemistry

Honors and Awards:
Henry Fok Young Teachers Award 2016 China
The National Science Fund for Excellent Young Scholars 2015 China
Program for New Century Excellent Talents in University 2014 China
The Hunan Province Science Fund for Distinguished Young Scholars 2014 Hunan Province
Award for Excellent Doctoral Student Granted by Ministry of Education 2010 China
Excellent Graduate Student 2010 Hunan University
Excellent Student 2005 Hunan University
2nd –Class Scholarship 2005 Hunan University
National Scholarship 2004 China
Triple A Student 2004 Hunan University
1st –Class Scholarship 2004 Hunan University
National Scholarship 2003 China
Triple A Student 2003 Hunan University
1st –Class Scholarship

Selected publications:
44. Ye, Z.; Wei, L.; Xiao, L.* Transient Binding-Assisted Circular Rocking Diffusion of Bacterium at the Liquid/Solid Interface Revealed by Single Particle Tracking. 2017

43. Shi, X.*; He, Y.; Gao, W.; Liu, X.; Ye, Z.; Liu, H.; Xiao, L.* Quantifying the Degree of Aggregation from Fluorescent Dye-Conjugated DNA Probe by Single Molecule Photobleaching Technology for the Ultra-sensitive Detection of Adenosine. Anal. Chem., 2017, submitted

42. Zhang, H.; Shang, Y.; Li, Y.; Xiao, L.; Yin, X.* Magnetic Resonance Imaging-Guided Stimulus-Responsive Release Targeted Chemotherapy with Glucose-Coated Nanoscale Metal-Organic Frameworks. Chem. Sci., 2017, submitted.

41. Zhang, P.; Wang, H.; Zhang, D.; Zeng, X.; Zeng, R.*; Xiao, L.*; Long, Y.; Yi, P.; Chen, J.* Two-photon Fluorescent Probe for Lysosome-Targetable Hypochlorous Acid Detection within Living Cells. Sens. Actuators B Chem., 2018, 255, 2223-2231.


40. Wang, Y.; Yang, Z.; Xiao, L.; Yin, X.* Lab-on-MOFs: Color-Coded Multi-Target Fluorescence Detection with White-Light Emitting Metal-Organic Frameworks under Single Wavelength Excitation. Chem. Comm., 2017, submitted.

39. Zhong, W.; Zeng, X.; Chen, J.*; Hong, Y.; Xiao, L.; Zhang, P.* Photoswitchable fluorescent polymeric nanoparticles for rewritable fluorescence patterning and intracellular dual-color imaging with AIE-based fluorogens as FRET donors. Polym. Chem., 2017, 8, 4849-4855.

38. Song, W.; Duan, W.; Liu, Y.; Ye, Z.; Chen, Y.; Chen, H.; Qi, S.; Wu, J.; Liu, D.; Xiao L.*; Ren, C.*;and Chen, X. Ratiometric Detection of Intracellular Lysine and pH with One-Pot Synthesized Dual Emissive Carbon Dots. 2017, Anal. Chem., 2017, 89, 13626-13633.

37. Ye, Z.; Wei, L..; Zeng, X..; Weng, R.; Shi, X.; Wang, N.; Chen, L.; Xiao L.* Background-Free Imaging of Viral Capsid Proteins-Coated Anisotropic Nanoparticle on Living Cell Membrane with Dark-Field Optical Microscopy. Anal. Chem., 2017, DOI: 10.1021/acs.analchem.7b03762.

36. Zhang, Z.; Zhang, D.; Wei, L.; Ma, M.*; Chen, B.; Xiao, L.* Temperature Responsive Fluorescent Polymer Nanoparticles (TRFNPs) for Subcellular Imaging and Controlled Releasing of Small Guest Molecules to Living Cells. Colloid. Surface B, 2017, 159, 905-912.

35. Wei, L.; Ma Y.; Zhu, X.; Xu, J.; Wang, Y.; Duan, H.; Xiao, L.* Sub-diffraction-Limit Localization Imaging of Plasmonic Nanoparticle Pair with Wavelength-Resolved Dark-Field Microscopy. Nanoscale, 2017, 9, 8747-8755.

34. Wei, L.; Zheng, X.; Ye, Z.; Xu, Y.; Zhang, D.; Xiao, L.* Positively Charged Small Size Fluorescent Conjugated Polymer Dots for Gene Delivery and Intracellular Imaging. Nanotheranostics, 2017, in press.

33. Tan, H.; Huang, Y.; Xu, J.; Chen, B.; Zhang, P.; Ye, Z,; Xiao, L.*; Liang, S.; Liu, Z.* The Enhanced Tumor-targeting Intracellular Translocation and Application of Lycosin-I Modified Gold Nanoparticles in Cancer Therapy. Theranostics, 2017, 7, 3168-3178.

32. Wei, L.; Ma, Y.; Shi, X.; Wang, Y.; Su, X.; Yu. C.; Xiang, S.; Xiao, L.*; Chen, B.* Living Cell Intracellular Temperature Imaging with Biocompatible Dye-Conjugated Carbon Dots. J. Mater. Chem. B, 2017, 5, 3383-3390.

31. Wang, J.; Xiong, G.; Ma, L.; Wang, S.; Zhou, X.; Wang, L.; Xiao, L.; Su, X.*; Yu, C.* A Dynamic Sandwich Assay on Magnetic Beads for Selective Detection of Single-Nucleotide Mutations at Room Temperature. Biosens. Bioelectron., 2017, 94, 305-311.

30. Wang, N.; Zhang, Y.; Lei, X.; Yu, W.; Zhan, Y.; Wang, D.; Zhang, J.; Wang, A.; Xiao, L.; Jiang, P.*; Yang, Y.* Optimized Conditions for Preserving Stability and Integrity of Porcine Circovirus Type2 Virus-like Particles during Long-term Storage. J. Virol. Methods, 2017, 243, 146-150.

29. Su, X.; Li, Z.; Yan, X.; Wang, L.; Zhou, X.; Wei, L.; Xiao, L.*; Yu, C.* Telomerase Activity Detection with Amplification-Free Single Molecule Stochastic Binding Assay. Anal. Chem., 2017, 89, 3576-3582.


28. Wei, L.; Ye, Z.; Xu, Y.; Chen, B.; Yeung, E. S.; Xiao, L.* Single Particle Tracking of Peptides-Modified Nanocargo on Lipid Membrane Reveals Bulk-Mediated Diffusion. Anal. Chem., 2016, 88, 11973-11977.

27. Poon, C.; Wei, L.; Xu, Y.; Chen, B.; Xiao, L.*; Li, H.* Quantification of Cancer Biomarkers in Serum Using Scattering-Based Quantitative Single Particle Intensity Measurement with a Dark-Field Microscope. Anal. Chem., 2016, 88, 8849-8856.

26. Ma, Y.; Zhang, Z.; Xu, Y.; Ma, M.; Chen, B.; Wei, L.; Xiao, L.* A Bright Carbon-dot-based Fluorescent Probe for Selective and Sensitive Detection of Mercury Ions. Talanta, 2016, 161, 476-481.

25. Luo, W.; Wu, M.; Li, S.; Xu, Y.; Ye, Z.; Wei, L.; Chen, B.; Xu; Q.; Xiao, L.* Nanoprecipitation of Fluorescent Conjugated Polymer onto the Surface of Plasmonic Nanoparticle for Fluorescence/Dark-field Dual-modality Single Particle Imaging. Anal. Chem., 2016, 88, 6827-6835.

24. Yang, Q.; Ye, Z.; Zhong, M.; Chen, Bo.; Chen, J.; Zheng, R.; Wei, L.*; Li, H.*; Xiao, L.* Self-Assembled Fluorescent Bovine Serum Albumin Nanoprobes for Ratiometric pH Measurement inside Living Cells. ACS Appl. Mater. Interfaces, 2016, 8, 9629-9634.

23. Wei, L.; Ye, Z.; Luo, W.; Xiao, L.* Redistribution of fluorescent molecules at the solid/liquid interface with total internal reflection illumination. Talanta, 2016, 155, 229-234.

22. Tan, H.; Luo, W.; Wei, L.; Chen, B.; Li, W.; Xiao, L.*; Manzhos, S.*; Liu, Z.*; Liang, S. Quantifying the Distribution of the Stoichiometric Composition of Anticancer Peptide Lycosin-I on the Lipid Membrane with Single Molecule Spectroscopy. J. Phy. Chem. B, 2016,120, 3081-3088.

21. Wei, L.; Xu, J.; Ye, Z.; Zhu, X.; Zhong, M.; Luo, W.; Chen, B.; Duan, H.; Liu, Q.; Xiao, L.* Orientational Imaging of Single Gold Nanorod at Liquid/solid Interface with Polarized Evanescent Field Illumination. Anal. Chem., 2016, 88, 1995-1999.


20. Yang, Q.; Wei, L.; Zheng, X.; Xiao, L.* Single Particle Dynamic Imaging and Fe3+ Sensing with Bright Carbon Dots Derived from Bovine Serum Albumin Proteins. Sci. Rep., 2015, 5, 17727.


19. Wei, L.; Zhou, P.; Yang, Q.; Yang, Q.; Ma, M.; Chen, B.; Xiao, L.* Fabrication of Bright and Small Size Semiconducting Polymer Nanoparticles for Cellular Labelling and Single Particle Tracking. Nanoscale, 2014, 6, 11351-11358.

18. Wei, L.; Yang, Q.; Xiao, L.* Tempo-spatially Resolved Cellular Dynamics of Human Immunodeficiency Virus Transacting Activator of Transcription (Tat) Peptide-modified Nanocargos in Living Cells. Nanoscale, 2014, 6, 10207-10215.

17. Li, H.; Guo, Y.; Xiao, L.*; Chen, B.* A fluorometric biosensor based on H2O2-sensitive nanoclusters for the detection of acetylcholine. Biosen. Bioelec., 2014, 59, 289-292.

16. Li, H.; Guo, Y.; Xiao, L.*; Chen, B.* Selective and sensitive detection of acetylcholinesterase activity using denatured protein-protected gold nanoclusters as a label-free probe. Analyst, 2014,139, 285-289.

15. Xiao, L.*; Yeung, E. S.* Optical Imaging of Individual Plasmonic Nanoparticles in Biological Samples. Annu. Rev. Anal. Chem., 2014, 7, 89-111.


14. Wei, L.; Zhao, X.; Chen, B.; Li, H.; Xiao, L.*; Yeung, E. S.* Frozen Translational and Rotational Motion of Human Immunodeficiency Virus Transacting Activator of Transcription Peptide-Modified Nanocargo on Neutral Lipid Bilayer. Anal. Chem., 2013, 85, 5169–5175.

13. Wei, L.; Liu, C.; Chen, B.; Zhou, P.; Li, H.; Xiao, L.*; Yeung, E. S.* Probing Single-Molecule Fluorescence Spectral Modulation within Individual Hotspots with Subdiffraction-Limit Image Resolution. Anal. Chem., 2013, 85, 3789-3793.

+++++++++++++++++++++Independent Work+++++++++++++++++++++++++++


12. Xiao, L.; Wei, L.; Liu, C.; He, Y.; Yeung, E. S.* Unsynchronized Translational and Rotational Diffusion of Nanocargo on a Living Cell Membrane. Angew. Chem. Int. Ed., 2012, 51, 4181-4184.

11. Xiao, L.; Ha, J. W.; Wang, G.; Wei, L.; Fang, N.* Determining the Full Three-Dimensional Orientation of Single Anisotropic Nanoparticles by Differential Interference Contrast Microscopy. Angew. Chem. Int. Ed., 2012, 51, 7734-7738.

10. Chan, H.-M.; Xiao, L.; Yeung, K.-M.; Ho, S.-L.; Zhao, D.; Chan, W.-H.; Li, H.-W.* Effect of surface-functionalized nanoparticles on the elongation phase of beta-amyloid (1-40) fibrillogenesis. Biomaterials, 2012, 33, 4443-4450.


9. Xiao, L.; Wei, L.; Cheng, X.; He, Y.*; Yeung, E. S. Noise-Free Dual-Wavelength Difference Imaging of Plasmonic Resonant Nanoparticles in Living Cells. Anal. Chem., 2011, 83, 7340-7347.

8. Xiao, L.; Qiao, Y.; He, Y.*; Yeung, E. S. Imaging Translational and Rotational Diffusion of Single Anisotropic Nanoparticles with Planar Illumination Microscopy. J. Am. Chem. Soc., 2011, 133, 10638-10645.

7. Wei, L.; Xiao, L.; He, Y.* Synthesis of water soluble silver-nanoparticle-embedded polymer nanofibers with poly(2-ethyl-2-oxazoline) by a straightforward polyol process. J. Mat. Res., 2011, 26, 1614-1620.

6. Sun, W.; Xiao, L.; Fang, N. Cell Imaging Techniques: Methods and Protocols, Methods in Molecular Biology, Human Press. Springer, Book Chapter, 2011.


5. Xiao, L.; Wei, L.; He, Y.*; Yeung, E. S. Single Molecule Biosensing Using Color Coded Plasmon Resonant Metal Nanoparticles. Anal. Chem., 2010, 82, 6308-6314.

4. Xiao, L.; Qiao, Y.; He, Y.*; Yeung, E. S.* Three Dimensional Orientational Imaging of Nanoparticles with Darkfield Microscopy. Anal. Chem., 2010, 82, 5268-5274.

3. Xiao, L.; Zhao, D.; Chan, W.-H.; Choi, M. M. F.; Li, H.-W.* Inhibition of beta 1–40 amyloid fibrillation with N-acetyl-L-cysteine capped quantum dots. Biomaterials, 2010, 31, 91-98.


2. Xiao, L.; Zhou, R.; He, Y.*; Li, Y.; Yeung, E. S.* Direct Observation of Nanoparticle Self-Assembly Dynamics at the Water-Air Interface Using Differential Interference Contrast Microscopy. J. Phys. Chem. C., 2009, 113, 1209-1216.

1. Xiao, L.; He, Y.*; Yeung E. S.* High Throughput Single Molecule Spectral Imaging of Photoactivated Luminescent Silver Clusters on Silver Island Films. J. Phys. Chem. C., 2009, 113, 5991-5997.