生物磁共振，蛋白质与多肽化学，抗体偶联，生物有机分析

1991年9月－1995年6月，曲阜师范大学，学士学位

1995年9月－1998年6月，南开大学，硕士学位

1998年9月－2001年6月，南开大学，博士学位

2001年8月－2004年6月，意大利佛罗伦萨大学-欧盟磁共振中心，博士后

2004年8月－2010年9月，澳大利亚国立大学，先后任博士后、研究员

2010年9月至今，南开大学化学学院，先后任特聘研究员、教授

长期从事生物磁共振和蛋白质化学研究。在蛋白质修饰的化学选择性、顺磁探针 的质量和顺磁效应的定量评价与分析方面做出了大量工作，发展了一系列较为实用的蛋白质定点标记方法和应用于细胞内磁共振研究的顺磁探针以及重要生物小分子的同时定量与细胞内测定的核磁方法。已在Acc Chem Res, Proc Natl Acad Sci USA, JACS, ACS Cent Sci和 Angew Chem Int Ed 等期刊发表论文 80 余篇。与美国、德国、以色列、澳大利亚和意大利等国家科学家有长期密切合作关系，其中与威兹曼科学研究院Daniella Goldfarb教授合作过程中连续获得两次国家自然科学基金委资助的国际合作项目。

针对高分辨阐述细胞内蛋白质及生物大分子动态互作与结构行为，发展蛋白质定点标记化学和生物磁共振方法。主要研究内容有：1）生物大分子定点（离体与细胞内）标记化学；2）原位环境下生物大分子动态结构与互作的高分辨磁共振研究方法；3）生物有机分析（核磁共振方法学）。

部分代表作：

1) Li M, Zhang Q*, Kong X, Zhao S, Pan BB, Sun Z, Yu P, Wang Z, Wang M, Ji W, Kong F, Cheng G, Wu S, Wang Y, Chen S, Su XC*, Shi F*. Quantum relaxometry for detecting biomolecular interactions with single NV centers. Proc Natl Acad Sci U S A. 2025, 122:e2509102122

2) Pan BB*, Zhou C, Meng WH, Zhang LY, Zhao JL, Su XC. Transsulfurase-catalyzed reversible modification of C-terminal cysteine and application for orthogonal dual labeling of proteins. Angew. Chem. Int. Ed. Engl. 2025, 64:e202507661.

3) Xu LH, Chen YT, Zhang X, Su XC*. In situ quantification of ammonia in biofluids using high-resolution nuclear magnetic resonance. Anal. Chem. 2025, 97, 12605-12612.

4) Cui CY, Li B, Zhang X, Guo SL, Pan BB, Su XC*. Visualization and quantification of base-level SO2 in live cells without intracellular background interference using sensitive 19F-NMR. J. Am. Chem. Soc. 2025, 147, 19893-19901

5) Zhang X, Li MH, Chen BG, Liu HK, Su XC*. Systematic kinetic assay of phenylsulfonyl pyridine derivatives with free thiol for site-specific modification of proteins. Org. Biomol. Chem. 2025, 23, 4768-4775

6) Li MH, Zhang X, Pan BB, Su XC*. Excellent Fe(II) binding tag in protein paramagnetic NMR spectroscopy. Inorg. Chem. 2025, 64, 7890-7901 

7) Chen JL, Chen SN, Liu HK, Pan BB, Zhao Y, Fu X, Otting G, Su XC*. Rational design of lanthanide-binding tags to optimize magnetic anisotropy in paramagnetic protein NMR. J. Am. Chem. Soc. 2025, 147, 9939-9952.

8) Guo SL, Pan BB, Li XY, Xiao YH, Su XC*. 19F-PCS measurements on proteins in live mammalian cells. Chem. Commun. 2025, 1156-1159.

9) Meng WH, Zhang X, Pan BB, Tan X, Zhao JL, Liu Y, Yang Y*, Goldfarb D, Su XC*. Efficient orthogonal spin labeling of proteins via aldehyde cyclization for pulsed dipolar EPR distance measurements. J. Am. Chem. Soc. 2025, 147, 234-246.

10) Chen JL, Li B, Yang F, Shi T, Su XC*. Increase of chiral sensing ability in host-guest chemistry by magnetic anisotropy. Since China Chem. 2024, 67, 2775-2782.

11) Chen JL, Wang X, Yang F, Li B, Otting G, Su XC*. 3D Structure of the transient intermediate of the enzyme–substrate complex of sortase A reveals how calcium binding and substrate recognition cooperate in substrate activation. ACS Catal. 2023, 13, 11610-11624

12) Cui CY, Li B, Su XC*. Real time monitoring of the level and activity of intracellular glutathione in live cells at atomic resolution by 19F-NMR. ACS Cent. Sci. 2023, 9, 1623-1632.

13) Pan BB, Liu HZ, Meng WH, Su XC*. Pt(IV) complex selectively oxidizes alpha-synuclein methionine as disclosed by NMR. Inorg. Chem. Front. 2023, 10, 296-304

14) Su XC*, Zhang LY, Zhao LN, Pan BB, Chen BG, Chen JL, Zhai CL, Li B. Efficient protein-protein couplings mediated by small molecules under mild conditions. Angew. Chem. Int. Ed. Engl. 2022, 61, e202205597

15) Chen YT, Li B, Chen JL, Su XC*. Simultaneous discrimination and quantification of enantiomeric amino acids under physiological conditions by chiral 19F NMR tag. Anal. Chem. 2022, 94, 7853-7860.

16) Wu X, Ma F*, Pan BB, Zhang Y, Zhu L, Deng F, Xu L, Zhao Y, Yin X, Niu H, Su XC*, Shi L*. Tailoring a nanochaperone to regulate α-synuclein assembly. Angew. Chem. Int. Ed. Engl. 2022, 61: e202200192

17) Cui CY, Li B, Cheng D, Li XY, Chen JL, Chen YT, Su XC*. Simultaneous quantification of biothiols and deciphering diverse GSH stability in different live cells by 19F-tag. Anal. Chem. 2022, 94, 901-908

18) Chen YT, Li B, Li XY, Chen JL, Cui CY, Hu K, Su XC*. Simultaneous identification and quantification of amino acids in biofluids by reactive 19F-tags. Chem. Commun. 2021, 13154-13157

19) Chen JL, Chen BG, Li B, Yang F, Su XC*. Assessing multiple conformations of lanthanide binding tags for proteins using a sensitive 19F‐reporter. Chem. Commun. 2021, 4291‐4294 

20) Chen JL, Li B, Li XY, Su XC*. Dynamic exchange of the metal chelating moiety: a key factor in determining the rigidity of protein‐tag conjugates in paramagnetic NMR. J. Phys. Chem. Lett. 2020, 11, 9493‐9500

21) Yang Y, Chen SN, Yang F, Li XY, Feintuch A, Su XC*, Goldfarb* D. In‐cell destabilization of a homodimeric protein complex detected by DEER spectroscopy. Proc. Natl. Acad. Sci USA 2020, 117, 20566‐20575 

22) Yang Y, Pan BB, Tan X, Yang F, Liu Y*, Su XC*, Goldfarb D*. In‐cell trityl‐trityl distance measurements on proteins. J. Phys. Chem. Lett. 2020, 11, 1141‐1147

23) 苏循成, 顺磁标记蛋白质在溶液与细胞内研究中的应用.中国科学‐化学 2019, 49, 1318‐1332

24) Chen JL, Wang X, Xiao YH, Su XC*. Resonance assignments of lowly populated and unstable enzyme intermediate complex under real‐time conditions. Chembiochem. 2019, 20, 2738‐2742.

25) Su XC*, Chen JL. Site‐specific tagging of proteins with paramagnetic ions for determination of protein structures in solution and in cells. Acc. Chem. Res. 2019, 52, 1675‐1686.

26) Yang Y, Yang F, Gong YJ, Bahrenberg T, Feintuch A, Su XC*, Goldfarb D*. High sensitivity in‐cell EPR distance measurements on proteins using an optimized Gd(III) spin label. J. Phys. Chem. Lett. 2018, 9, 6119‐6123

27) Yang Y, Yang F, Gong YJ, Chen Jl., Goldfarb D*, Su XC*. A reactive, rigid Gd(III) labelling tag for in‐cell EPR distance measurements in proteins. Angew. Chem. Int. Ed. Engl. 2017, 56, 2914‐2918.

28) Ma FH, Wang X, Chen JL, Wen X, Sun H*, Su XC*. Deciphering the multisite interactions of a protein and its ligand at atomic resolution by using sensitive paramagnetic effects. Chem. Eur. J. 2017, 23, 926‐934.

29) Chen JL, Wang X, Yang F, Cao C, Otting G, Su XC*. 3D Structure determination of an unstable transient enzyme intermediate by paramagnetic NMR spectroscopy. Angew. Chem. Int. Ed. Engl. 2016, 55, 13744‐13748.

30) Pan BB, Yang F, Ye Y, Wang JT, Li C*, Huber T*, Su XC*. Structural determination of proteins in living cells by paramagnetic NMR spectroscopy. Chem. Commun. 2016, 52, 10237‐10240.

已培养博士20名，硕士22名等